Centrifuge at 10,000–15,000 x g for 5–15 min at 4 C. Carefully decant the supernatant without disturbing the pellet. Air-dry the pellet for 5–20 min (depending on the size of the pellet). Redissolve the DNA in a suitable buffer. Tip: Use a buffer with a pH of 7.5–8.0, as DNA does not dissolve easily in acidic buffers.
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Get DetailsFor analysis of raw oysters, include a second tared flask with 25 g of product plus 2475 ml APW. This flask should be incubated 18 to 21 h at 42 0.2 C in a water bath (28,31).
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Get DetailsMar 17, 2022 From 2015 to 2021, we optimized mass cultivation of diatoms in our own developed vertical column airlift photobioreactors using natural and artificial light (LEDs). The project took place at the ferrosilicon producer Finnfjord AS in North Norway as a joint venture with UiT—The Arctic University of Norway. Small (0.1–6–14 m3) reactors were used for initial …
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Get DetailsThe University of Colorado Boulder (CU Boulder, CU, or Colorado) is a public research university in Boulder, Colorado.Founded in 1876, five months before Colorado became a state, it is the flagship university of the University of Colorado system. CU Boulder is a member of the Association of American Universities, a selective group of major research universities in North …
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