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Bone Marrow-Derived Macrophages (BMM): Isolation and ...

Bone Marrow-Derived Macrophages (BMM): Isolation and ...

Bone marrow-derived macrophages (BMM) are primary macrophage cells, derived from bone marrow cells in vitro in the presence of growth factors. Macrophage colony-stimulating factor (M-CSF) is a lineage-specific growth factor that is responsible for the proliferation and differentiation of committed myeloid progenitors into cells of the ...

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The truth about fetal tissue research | Nature

The truth about fetal tissue research | Nature

Dec 07, 2015 Prominent among these is the BLT (bone marrow–liver–thymus) mouse, which was created in 2006 2. This model is made by destroying the animal’s immune system and then surgically transplanting ...

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The Truth about Fetal Tissue Research - Scientific American

The Truth about Fetal Tissue Research - Scientific American

Dec 09, 2015 The Truth about Fetal Tissue Research. The use of aborted fetal tissue has sparked controversy in the U.S., but many scientists say it is essential for studies of HIV, development and more

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Induction of osteogenesis by bone-targeted Notch ...

Induction of osteogenesis by bone-targeted Notch ...

Feb 04, 2022 Notch activity in bone marrow (BM) macrophages inhibits commitment to osteoclast differentiation, but it enhances the maturation and function of committed osteoclast precursors (Jim nez-Alc zar et al., 2017; Wu et al., 2010). Notch is generally a negative regulator of endothelial cell (EC) proliferation and vascular growth, but exerts the ...

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Chromosome Preparation From Cultured Cells - PMC

Chromosome Preparation From Cultured Cells - PMC

Jan 28, 2014 Chromosome studies are also used to assess disease progression such as the onset of blast crisis and response to treatment. Since bone marrow cells are actively dividing, no mitogen stimulation is necessary 9,19. For acute leukemias 24 and 48 hr cultures are also set up. Collect at least 1 ml of bone marrow in a green top sodium heparin tube.

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Isolation, Purification and Labeling of Mouse Bone Marrow ...

Isolation, Purification and Labeling of Mouse Bone Marrow ...

Jul 10, 2013 Second, mouse bone marrow neutrophils have been shown to survive protracted periods of time in culture, significantly longer compared to mouse blood neutrophils 16. Therefore, harvesting of bone marrow over blood neutrophils could allow for greater yield of cells when processed for functional assays ex vivo. Third, isolation of neutrophils from ...

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Preclinical Evaluation of BMP-9-Treated Human Bone-like ...

Preclinical Evaluation of BMP-9-Treated Human Bone-like ...

Mar 18, 2022 The success of dental implant treatment after tooth extraction is generally maximized by preserving the alveolar ridge using cell-free biomaterials. However, these treatments can be associated with inflammatory reactions, leading to additional bone volume loss hampering dental implant positioning. Our group developed a self-assembled bone-like …

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An aged immune system drives senescence and ... - Nature

An aged immune system drives senescence and ... - Nature

May 12, 2021 Bone marrow was stained with CD19-APC, B220-FITC, F4/80-PE-Cy7 and CD11b-PE purchased from either BD Pharmagin or eBioscience) to sort B cells and macrophages. Cells (5 10 4 ) were sorted into ...

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Water extract of Cimicifugae rhizoma reprogramming ...

Water extract of Cimicifugae rhizoma reprogramming ...

Mouse Bone Marrow-Derived Macrophages (BMDMs) Preparation and Polarization ... The centrifuge was set at 4 C and 300 g for 10 min and the supernatant was discarded to obtain tumor tissue single cells. Cells were resuspended in 450 μL pre-cooled 1 PBS and 50 μL MASC F4/80+ microbeads were added and mixed. We placed it on a 4 C freezer and ...

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Cell Surface Flow Cytometry Staining Protocol - BioLegend

Cell Surface Flow Cytometry Staining Protocol - BioLegend

Obtain desired tissue (e.g. spleen, lymph node, thymus, bone marrow) and prepare a single cell suspension in Cell Staining Buffer (BioLegend Cat. No. 420201). If using in vitro stimulated cells, simply resuspend previously activated cultures in Cell Staining Buffer and proceed to Step 2.

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