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University of Leeds

University of Leeds

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Bone Marrow-Derived Macrophages (BMM): Isolation and ...

Bone Marrow-Derived Macrophages (BMM): Isolation and ...

Alternatively, separate the femur and tibia by cutting at the knee joint. Flush the bones with lymphocyte medium using a 5-mL syringe and a 25-gauge needle. 9. Pipet the bone marrow cells up and down to bring the cells into single-cell suspension. 10. Pass the cells through a …

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Marginal zone B cells acquire dendritic cell functions by ...

Marginal zone B cells acquire dendritic cell functions by ...

Bone marrow (BM) was harvested from tibia and femur of donor mice (wild-type, Marchf1 –/–, and Cr2 –/–), and recipient mice (wild-type and C3 –/–) were irradiated twice at 550 cGy (rad), 3 hours apart, before intravenously injected with 50:50 mixed BM cells. Recipient mice were intraperitoneally injected with anti-Thy1 (clone T24 ...

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Combined single-cell and spatial ... - Nature Cell Biology

Combined single-cell and spatial ... - Nature Cell Biology

Dec 23, 2019 The bone marrow constitutes the primary site for life-long blood production and skeletal regeneration. However, its cellular and spatial organization remains controversial. Here, we combine single ...

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Full text of "NEW"

Full text of "NEW"

Due to a planned power outage on Friday, 1/14, between 8am-1pm PST, some services may be impacted.

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Induction of osteogenesis by bone-targeted Notch ...

Induction of osteogenesis by bone-targeted Notch ...

Feb 04, 2022 Notch activity in bone marrow (BM) macrophages inhibits commitment to osteoclast differentiation, but it enhances the maturation and function of committed osteoclast precursors (Jim nez-Alc zar et al., 2017; Wu et al., 2010). Notch is generally a negative regulator of endothelial cell (EC) proliferation and vascular growth, but exerts the ...

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Hypertrophic chondrocytes serve as a reservoir for marrow ...

Hypertrophic chondrocytes serve as a reservoir for marrow ...

Feb 18, 2022 Hypertrophic chondrocytes of the growth plate undergo a process of dedifferentiation to generate marrow associated skeletal stem and progenitor cells (SSPCs), which ultimately re-differentiate into cells of the osteoblast …

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Isolation, Purification and Labeling of Mouse Bone Marrow ...

Isolation, Purification and Labeling of Mouse Bone Marrow ...

Jul 10, 2013 Representative FACS plot of isolated neutrophils from bone marrow cells of an uninfected C57BL/6 mouse using the density gradient centrifugation protocol. The left panel demonstrates the initial gating used for the bone marrow cells collected from the interface of Histopaque 1119 and Histopaque 1077 using forward and side scatter (FSC/SSC).

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TRPM7 kinase-mediated ... - Nature Communications

TRPM7 kinase-mediated ... - Nature Communications

May 17, 2021 Deficiency in Mg 2+ is known to disrupt systemic bone metabolism, characterized by the inadequate bone formation and deregulated bone resorption 2,3,4,5,6,7,8. In contrast, Mg 2+ supplement is ...

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Bone substitutes in orthopaedic surgery: from basic ...

Bone substitutes in orthopaedic surgery: from basic ...

May 28, 2014 Autografts. Currently autografts are the “gold standard” in bone substitution [13, 14].Autologous (or autogenous) bone grafting involves utilizing bone obtained from the same individual receiving the graft [].Bone can be harvested from non-essential bones, such as the iliac crest or the fibula (Fig. 1), the chin, the ribs, the mandible and even parts of the skull.

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Extrapulmonary Tuberculosis: An Overview - American Family ...

Extrapulmonary Tuberculosis: An Overview - American Family ...

Nov 01, 2005 A chest radiograph or CT scan reveals numerous 2- to 3-mm nodules scattered throughout the lung in more than 85 percent of patients .16, 39 Common laboratory abnormalities include normochromic ...

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