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Migrasomes provide regional cues for ... - Nature Cell Biology

Migrasomes provide regional cues for ... - Nature Cell Biology

Aug 01, 2019 Zhang, J., Jiang, Z., Liu, X. & Meng, A. Eph/ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer ...

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Centrifugation - Wikipedia

Centrifugation - Wikipedia

Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor speed. The denser components of the mixture migrate away from the axis of the centrifuge, while the less dense components of the mixture migrate towards the axis.

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Coenzyme Q10 restores oocyte mitochondrial function and ...

Coenzyme Q10 restores oocyte mitochondrial function and ...

Jun 26, 2015 After centrifugation at 9000 g for 10 min, the hexane layer was collected and evaporated to dryness using a vacuum centrifuge (Eppendorf, Mississauga, ON, Canada). The quinone residue was then dissolved in 100% ethanol and analyzed by HPLC with UV detection at 275 nm (Beckman System Gold, Beckman Coulter Inc, Brea, CA, USA).

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A critical role of hepatic GABA in the ... - cell.com

A critical role of hepatic GABA in the ... - cell.com

Jun 29, 2021 Geisler et al. show that GABA-transaminase catalyzes GABA synthesis in the liver of obese mice, resulting in hyperinsulinemia, insulin resistance, and hyperphagia. In people with obesity, liver GABA-transaminase expression is positively associated with hyperinsulinemia. Thus, GABA-transaminase inhibitors may be effective at restoring glucose homeostasis in obese, …

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Pro- and anti-apoptotic microRNAs are differentially ...

Pro- and anti-apoptotic microRNAs are differentially ...

Mechanisms that encourage cell survival and inhibit the apoptosis response are especially important during X. laevis estivation as the frog can spend months in its burrow during drought periods. This inactivity can result in severe muscle wasting in non-adapted animals, as well as atrophy of liver and intestinal cells due to the unavailability ...

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Myc-Tag (71D10) Rabbit mAb | Cell Signaling Technology

Myc-Tag (71D10) Rabbit mAb | Cell Signaling Technology

Microcentrifuge for 10 min at 4 C, 14,000 x g and transfer the supernatant to a new tube. The supernatant is the cell lysate. If necessary, lysate can be stored at -80 C. C. Immunoprecipitation Cell Lysate Pre-Clearing (Optional) Vortex to mix beads. Add 10–30 l of 50% Protein A agarose bead slurry to 200 l cell lysate at 1 mg/ml.

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Subcellular Fractionation - labome.com

Subcellular Fractionation - labome.com

Nov 14, 2021 The cell lysate in 2 M sucrose was layered at the bottom of the discontinuous sucrose gradient. After centrifugation at 100,000 g for 1 h, the early stage melanosomes, which were recovered in the zone of about 1 M sucrose was then layered in the middle of an extended gradient of 0.8, 1.0, and 1.2 M sucrose and centrifuged again as before.

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Measurement of Cell Death in Mammalian Cells - PMC

Measurement of Cell Death in Mammalian Cells - PMC

Sep 01, 2004 During the process of cell isolation it is possible for artifacts of cell damage to be introduced. This could result in a mischaracterization of cytotoxicity. Additionally, many of the methods listed on Table 12.8.3 are not sensitive enough to determine the cytotoxic effects of compounds on tissues or in organs with differing volumes or cell types.

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Multiple early factors anticipate post-acute COVID-19 ...

Multiple early factors anticipate post-acute COVID-19 ...

T cell clonal dynamics, as inferred from T cell receptor (TCR) gene sequences and sc transcriptomics, can provide insights into the evolution of the adaptive immune response over the course of infection and recovery. We used TCR genes as barcodes to track the T2 to T3 dynamics of CD8 + and CD4 + T cell clonotypes (Figure 3A).

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