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Comprehensive analysis of spatial architecture in primary ...

Comprehensive analysis of spatial architecture in primary ...

Dec 17, 2021 After adding 10 ml of PBS and mixing thoroughly, the mixed solution was slowly added to the surface of the Ficoll solution (GE Healthcare, catalog no. 17544203). After centrifugation (800g for 20 min at

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Cell culture and cell analysis - Autoimmunity - NCBI Bookshelf

Cell culture and cell analysis - Autoimmunity - NCBI Bookshelf

Density gradient centrifugation. Barrier methods (centrifugation through a continuous centrifugation media). ... Using Ficoll-Hypaque, which has a density of 1.077 g/ mL, density identical to that of lymphocytes and monocytes, it is possible to recover PBMCs. The Ficoll-Hypaque is a combination of a polymer of high molecular weight sucrose ...

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Neutrophil Overview | Thermo Fisher Scientific - US

Neutrophil Overview | Thermo Fisher Scientific - US

In particular, the occurrence of low-density neutrophils (LDN) is markedly increased in inflammation or cancer. While most neutrophils in density gradient centrifugation of whole blood settle below the Ficoll layer (Normal Density Neutrophils/NDN), LDN sediment in the same fraction as mononuclear cells and comprise a mix of mature and immature ...

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Structural basis of a novel PD-L1 ... - Cell Discovery

Structural basis of a novel PD-L1 ... - Cell Discovery

Mar 07, 2017 The use of antibodies to target immune checkpoints, particularly PD-1/PD-L1, has made a profound impact in the field of cancer immunotherapy. Here, we identified KN035, an anti-PD-L1 nanobody that ...

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Collection, Storage, and Preparation of Human Blood Cells ...

Collection, Storage, and Preparation of Human Blood Cells ...

May 01, 2016 ISOLATION OF PERIPHERAL BLOOD MONOCYTES BY GRADIENT CENTRIFUGATION WITH FICOLL-HYPAQUE FOLLOWED BY PERCOLL. Peripheral blood monocytes are isolated from blood by centrifugation through a Ficoll-Hypaque or Histopaque gradient (Denholm and Wolber, 1991) followed by Percoll.

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A Review on Centrifugation in the Pharmaceutical Industry

A Review on Centrifugation in the Pharmaceutical Industry

May 02, 2015 Density gradient centrifugation is the preferred method to purify subcellular organelles and macromolecules. Density gradients can be generated by placing layer after layer of gradient media such as sucrose in a tube with the heaviest layer at the bottom and the lightest at the top in either a discontinuous mode. ... These include: Ficoll ...

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Peripheral blood mononuclear cells: PBMC isolation ...

Peripheral blood mononuclear cells: PBMC isolation ...

May 30, 2017 PBMCs are isolated by density gradient centrifugation, as different components of the blood have different densities and can be separated accordingly. The density gradient medium most commonly used (Ficoll or Ficoll-Paque) contains sodium diatrizoate, polysaccharides, and water, and has a density of 1.08 g/mL.

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Subcellular Fractionation - labome.com

Subcellular Fractionation - labome.com

Nov 14, 2021 Nuclei are removed by low-speed centrifugation, while the cytoplasm-containing supernatant is centrifuged in a fixed-angle rotor at 6500 g to pellet mitochondria. Additionally, there are protocols that utilize density-gradient separations which provide purer mitochondrial fractions, but they are more time-consuming and are avoided.

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Cytotoxic T cells are able to efficiently eliminate cancer ...

Cytotoxic T cells are able to efficiently eliminate cancer ...

Sep 01, 2021 At high CTL density (ET 1 : ... PMP-38) and maintained for further 24–48 h. Cells were collected on days 4 or 5 using Ficoll gradient …

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Marginal zone B cells acquire dendritic cell functions by ...

Marginal zone B cells acquire dendritic cell functions by ...

Splenic B cells were isolated from whole-splenocyte suspensions through gradient centrifugation (at 2237g) with Ficoll-Paque Plus (GE Healthcare) and subsequent negative depletion using FITC-conjugated mAb against CD4 (GK1.5, 1.6 μg/ml), Ly76 (TER119, 1.6 μg/ml), and CD43 (S7, 1.6 μg/ml) and magnetic anti-FITC MicroBeads (2 μl/10 6 cells ...

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