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Phenol-Chloroform Extraction - an overview | ScienceDirect ...

Phenol-Chloroform Extraction - an overview | ScienceDirect ...

Apr 01, 2010 Following centrifugation, the aqueous phase containing the purified DNA can be transferred to a clean tube for analysis. ... Incubate the exosome with 450 ... Cell pellets were suspended in 800 μl cold TRIzol and the procedure followed …

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Exosomes in clinical trial and their production in ...

Exosomes in clinical trial and their production in ...

Dec 05, 2019 The differential centrifugation is the common strategy for the concentration of CM and exosome isolation from the concentrated CM. Although a less additive reagent is added during differential centrifugation comparing to that of sucrose gradient method, workforce, and labor are the disadvantages of the differential centrifugation purification .

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ICAM-1-mediated adhesion is a prerequisite for exosome ...

ICAM-1-mediated adhesion is a prerequisite for exosome ...

Feb 07, 2022 ICAM-1 co-fractionated with PD-L1 and other known exosome marker proteins, including Alix, CD63, and Tsg101, as analyzed by iodixanol density gradient centrifugation (Figure 1C). Interferon-γ (IFN-γ) secreted by activated T cells upregulates tumor-cell-surface ICAM-1 ( Hama et al., 2008 ; Kim et al., 1995 ).

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Exosome detection via the ultrafast-isolation system ...

Exosome detection via the ultrafast-isolation system ...

Jan 11, 2021 The detailed step-by-step EXODUS protocol for exosome isolation from different biofluids including urine, cell culture medium, human saliva, ...

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A comparison of methods for the ... - Scientific Reports

A comparison of methods for the ... - Scientific Reports

Jan 23, 2020 After centrifugation at 14000 g for 5 min the supernatant was removed and the pellet was washed twice with cold acetone. ... Optimized exosome isolation protocol for cell culture supernatant and ...

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Progress in Exosome Isolation Techniques - PMC

Progress in Exosome Isolation Techniques - PMC

Jan 26, 2017 Lobb RJ, Becker M, Wen SW, Wong CSF, Wiegmans AP, Leimgruber A, M ller A. Optimized exosome isolation protocol for cell culture supernatant and human plasma. J. Extracell. Vesicles. 2015; 4:27031. [PMC free article] [Google Scholar]

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Streptavidin-coupled Dynabeads, Streptavidin Magnetic ...

Streptavidin-coupled Dynabeads, Streptavidin Magnetic ...

No tedious centrifugation, precipitation, filtration, or columns. Magnetic handling enables easy washing, separation, and concentration of your target. Excellent dispersion abilities and the lack of magnetic remanence make Dynabeads Streptavidin products ideal for manual as well as automated protocols, including microfluidic systems.

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Exosome: A Review of Its Classification, Isolation ...

Exosome: A Review of Its Classification, Isolation ...

Sep 22, 2020 In contrast, the density gradient centrifugation has an advantage in exosome purity, but the high viscosity of sucrose solution will reduce the sedimentation rate of exosomes, ... Evtushenko EG, et al. Isolation of exosomes by differential centrifugation: theoretical analysis of a commonly used protocol.

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D ynabeads magnetic beads - Thermo Fisher Scientific

D ynabeads magnetic beads - Thermo Fisher Scientific

The Dynabeads magnetic separation protocol generally consists of three simple steps. No centrifugation steps or columns are required for sample processing, and the protocol can be automated on Thermo Scientific™ KingFisher™ instruments. Dynabeads Target protein Nonspecific protein Antibody Versatility of the Dynabeads magnetic beads

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RNA | Kits | Bioanalysis - MACHEREY-NAGEL

RNA | Kits | Bioanalysis - MACHEREY-NAGEL

This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the comfort when using this website, are used for direct advertising or to facilitate interaction with other websites and social networks, are …

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