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Purification of proteins fused to glutathione S-tranferase ...

Purification of proteins fused to glutathione S-tranferase ...

1. Introduction. Glutathione S-transferase (GST) is a naturally occurring 26 KDa protein found in eukaryotic cells. The gene from the parasitic helminth Schistosoma japonicum was used in the development of the pGEX vectors ().This unit describes the use of a GST affinity tag to aid in the purification of recombinant proteins.

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Vacuum flask - Wikipedia

Vacuum flask - Wikipedia

A vacuum flask (also known as a Dewar flask, Dewar bottle or thermos) is an insulating storage vessel that greatly lengthens the time over which its contents remain hotter or cooler than the flask's surroundings. Invented by Sir James Dewar in 1892, the vacuum flask consists of two flasks, placed one within the other and joined at the neck.The gap between the two flasks is …

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Labware - Sigma-Aldrich

Labware - Sigma-Aldrich

Browse our selection of essential lab equipment, including glassware, multiwell plates, cell culture supplies, laboratory sampling and labelling supplies, …

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DMSO Hybri-Max, sterile-filtered CAS No. 67-68-5 Sigma-Aldrich

DMSO Hybri-Max, sterile-filtered CAS No. 67-68-5 Sigma-Aldrich

Dimethyl sulfoxide (DMSO); Hybri-Max, sterile-filtered, BioReagent, hybridoma, = 99.7%; Suitable for hybridoma cell culture; DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for …

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(PDF) Microgreens: Assessment of Nutrient Concentrations

(PDF) Microgreens: Assessment of Nutrient Concentrations

Fresh tissue (3 g) was weighed into a 50 mL centrifuge tube, and 10 mL of ice-cold 5% (w/v) metaphosphoric acid was added, followed by homogenization at the speed of 15 000 rpm

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r - How to determine which distribution fits my data best ...

r - How to determine which distribution fits my data best ...

Jan 08, 2015 I have a dataset and would like to figure out which distribution fits my data best. I used the fitdistr() function to estimate the necessary parameters to describe the assumed distribution (i.e. Weibull, Cauchy, Normal). Using those parameters I can conduct a Kolmogorov-Smirnov Test to estimate whether my sample data is from the same distribution as my …

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Amicon® Ultra-15 Centrifugal Filter Units - Sample Prep ...

Amicon® Ultra-15 Centrifugal Filter Units - Sample Prep ...

Milli-Q IQ 7003/05/10/15 ... world class partners EMD Millipore + Sigma-Aldrich = MilliporeSigma the life science business of Merck KGaA, Darmstadt, Germany ... Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 ...

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Amicon® Ultra Centrifugal Filters | Life Science Research ...

Amicon® Ultra Centrifugal Filters | Life Science Research ...

Milli-Q IQ 7003/05/10/15 ... world class partners EMD Millipore + Sigma-Aldrich = MilliporeSigma the life science business of Merck KGaA, Darmstadt, Germany. Our new packaging We are rebranding all of our life science products, labels, packages and documents. ... Centrifuge and Rotor Recommendations ...

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L-Arginine Modulates T Cell Metabolism and Enhances ...

L-Arginine Modulates T Cell Metabolism and Enhances ...

Oct 20, 2016 Oligomycin (1.4 μM, Sigma), Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP, 0.6 μM, Sigma) and antimycin (1.4 μM, Sigma) were injected. IL-2 Withdrawal Assay Naive CD4 T cells were activated with plate-bound CD3 and CD28 antibodies. 48 hr after activation IL-2 was added to culture media (500 U ml −1 ).

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Home - Restek Chromatography Products and Solutions

Home - Restek Chromatography Products and Solutions

Restek develops and manufactures GC and LC columns, reference standards, sample prep materials, and accessories for the international chromatography industry. Our unbeatable Plus 1 customer service and top-quality products are known world-wide.

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Disc Stack Centrifuge - an overview | ScienceDirect Topics

Disc Stack Centrifuge - an overview | ScienceDirect Topics

The sigma factor represents the equivalent area of the centrifuge and is unique for each disc stack centrifuge and the angular velocity. For continuous operation, the ratio between flow rate through the centrifuge, Q, and the sigma factor should be kept constant during the scale-up.

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Pi Bond - Definition, Explanation, Examples with Illustrations

Pi Bond - Definition, Explanation, Examples with Illustrations

The sp 2 hybridized carbons now form three sigma bonds and one pi bond, as illustrated below. The sp 2 hybridized orbital in the carbon atom is made up of a 2s electron, a 2p x electron, and a 2p y electron. It can form a total of three sigma bonds. The 2p z electrons of the carbon atoms now form a pi bond with each other. Thus, each carbon ...

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Water Content (w) - Geoengineer.org

Water Content (w) - Geoengineer.org

The water content (w), also known as natural water content or natural moisture content, is the ratio of the weight of water to the weight of the solids in a given mass of soil.This ratio is usually expressed as percentage. When voids are completely …

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agriGO: GO Analysis Toolkit and Database for Agricultural ...

agriGO: GO Analysis Toolkit and Database for Agricultural ...

Welcome to agriGO -- GO Analysis Toolkit and Database for Agricultural Community. The agriGO is a web-based tool and database for the gene ontology analysis.

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