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Bicinchoninic acid (BCA) assay or Smith assay is a copper-based colorimetric assay for total protein quantification. BCA rely on the formation of a Cu 2 +–protein complex in a basic environment, followed by reduction of the Cu 2 + to Cu + (Smith et al., 1985).The amount of Cu 2 + that is reduced is proportional to the amount of protein present in solution.
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determine the mass of the cup, stir bar, and water. Record this mass to 0.01 g on the data sheet. Determine the mass of water by subtraction. 3. On a triple-beam balance, determine the mass of an aluminum weighing dish. Add to the weighing dish a sample of potassium nitrate whose mass is in the range of 2.0 to 2.5 g. Record the mass of the ...
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Distillation, or classical distillation, is the process of separating the components or substances from a liquid mixture by using selective boiling and condensation. Dry distillation is the heating of solid materials to produce gaseous products (which may condense into liquids or solids). Dry distillation may involve chemical changes such as destructive distillation or cracking and is …
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j. Magnetic stirrer and stir bar (optional). k. Blender or homogenizer (optional) l. Beaker, low-form Class B or Class A having a volume sufficient enough to fully contain the sample and prevent sample loss from spillage or splattering when mixing. m. Glass-fiber filter, with a 47 mm diameter, nominal pore size ≤ 2.0 m
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Jul 21, 2021 LAB: To get started you will need a standard pH meter, a pH electrode, an ATC probe, a stirrer with stir bar, beaker, deionized water, and buffers. Method B If the testing is being performed for recreational gardening, you can use a pH tester with ATC (35634-15) and buffers for calibration (35653-04). Soil Sampling
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Jun 02, 2019 Phosphate buffered saline (PBS) is a buffer solution that's commonly used for immunohistochemical (IHC) staining and it's often used in biological research. PBS is a water-based salt solution containing sodium hydrogen phosphate, sodium chloride and, in some cases, potassium chloride and potassium dihydrogen phosphate.
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MAGNETIC BAR (MANYETİK BAR) Magnetic bars employ rotating magnetic field to mix a chemical mixture or a reaction continuously once they are placed in. For a magnetic bar to work a stirrer must be placed below or a heater with a stirrer feature. HEATER (ISITICI) A heater is a device that heats water or other solutions to a desired temperature.
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magnetic stirrer and bar; Three 100 mL graduated cylinders; 400 mL beaker; Procedure. Measure out 100 mL of each solution into graduated cylinders. With stirring, quickly add each solution to the 400 mL beaker. The solution will oscillate between colorless, amber and dark blue. Clock will oscillate for about 5 minutes typically.
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Magnetic Stirrer; Stir Bar; 100ml Graduated Cylinder (optional for pH measurement) After calibrating your meter with the buffers, rinse the electrode(s) and glassware with distilled or deionized water. Carefully measure 100 ml of your sample and place in a 150 ml beaker for the pH and alkalinity part. Place the rinsed electrode in the test sample.
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Nov 13, 2013 Assemble the calorimeter apparatus, as shown in Fig. 5, by positioning the cup containing the NaOH solution and stir bar on the magnetic stirrer. Your instructor will assist you in positioning the cover, if needed. Begin gently stirring the solution (a setting of 1 or 2 on the magnetic stirrer is a good starting point).
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Sep 22, 2021 Put the magnetic stirrer onto your buret stand so that the buret is directly over the magnetic stirrer. Place the magnetic stir-bar into the solution in the beaker labeled “A - ”. and set this beaker on top of the magnetic stirrer. Plug in the magnetic stirrer and set it to the lowest setting where the solution is mixing smoothly but gently.
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Steps in the procedure. Measure out 50 cm 3 of 1.0 mol dm-3 ethanoic acid using a measuring cylinder and transfer it to a 200 cm 3 beaker; Place the beaker onto a magnetic stirrer and add a magnetic stirrer bar; Position the pH probe so that it does not …
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Stirrer, magnetic, portable, 356FLD * Magnetic stirring bars, small, H-08546-00 # ... (herbicides). The capillary-column gas chromatography/mass spectrometry (GCMS) method is used for pesticide analyses of organonitrogen herbicides (NWQL schedules 2001 and 2010). ... thermistor, a small Teflon-coated magnetic stirring bar, and a glass beaker ...
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The rate of stirring of the magnetic stirrer should be kept constant. It is recommended to keep the rate at 600 revolutions per minute or switch to medium if the magnetic stirring plate does not have specific numbers on the revolution rate. The molar mass of iodine is used in the calculations. It should be constant, e.g. 126.9 u.
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